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mouse anti akap9 (Novus Biologicals)

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Novus Biologicals mouse anti akap9
Mouse Anti Akap9, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+akap9/pm28192412-478-0-7?v=Novus+Biologicals
Average 86 stars, based on 1 article reviews

mouse anti akap9 - by Bioz Stars, 2026-08

86/100 stars

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( A–B ) Western blot analysis of ( A ) AKAP6 or ( B ) PCM1 expression levels upon siRNA-mediated depletion of AKAP6. Loading control: α-tubulin. ( C ) Immunostaining of nesprin-1α (green), AKAP6 (red), and DNA (DAPI) in siControl- or si-AKAP6-depleted P3 cardiomyocytes. ( D ) Quantification of C as nuclear intensity normalized to the mean intensity in siControl. Error bars represent SD. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. **** p<0.0001, n.s.: no significance. n = 70, 83, 66, 98 (from left to right), data are from two independent experiments. ( E–H ) Immunostaining of ( E ) Pcnt (red) or ( G ) AKAP9 (red), cardiac troponin I (magenta, cardiomyocyte-specific), and DNA (DAPI) in AKAP6-depleted P3 cardiomyocytes transfected with an siRNA-resistant silent mutant of AKAP6 fused to GFP (AKAP6res-GFP). Transfected cells are denoted with a green arrowhead. ( F–H ) Quantification of E and G respectively, as nuclear intensity normalized to the mean intensity in siControl. Error bars represent SD. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. **** p<0.0001, n.s.: no significance. ( F ) n = 100 per condition, ( H ) n = 128, 133, 112 (from left to right), data are from three independent experiments. Scale bars: 10 µm.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A–B ) Western blot analysis of ( A ) AKAP6 or ( B ) PCM1 expression levels upon siRNA-mediated depletion of AKAP6. Loading control: α-tubulin. ( C ) Immunostaining of nesprin-1α (green), AKAP6 (red), and DNA (DAPI) in siControl- or si-AKAP6-depleted P3 cardiomyocytes. ( D ) Quantification of C as nuclear intensity normalized to the mean intensity in siControl. Error bars represent SD. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. **** p<0.0001, n.s.: no significance. n = 70, 83, 66, 98 (from left to right), data are from two independent experiments. ( E–H ) Immunostaining of ( E ) Pcnt (red) or ( G ) AKAP9 (red), cardiac troponin I (magenta, cardiomyocyte-specific), and DNA (DAPI) in AKAP6-depleted P3 cardiomyocytes transfected with an siRNA-resistant silent mutant of AKAP6 fused to GFP (AKAP6res-GFP). Transfected cells are denoted with a green arrowhead. ( F–H ) Quantification of E and G respectively, as nuclear intensity normalized to the mean intensity in siControl. Error bars represent SD. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. **** p<0.0001, n.s.: no significance. ( F ) n = 100 per condition, ( H ) n = 128, 133, 112 (from left to right), data are from three independent experiments. Scale bars: 10 µm.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Western Blot, Expressing, Immunostaining, Transfection, Mutagenesis

( A–B ) Immunostaining of ( A ) Cdk5Rap2 (green) or ( B ) Pcnt (green) and AKAP9 (red) together with cardiac troponin I (magenta, cardiomyocyte-specific) and DNA (DAPI) in rat P3 cardiomyocytes transfected with control-siRNA or AKAP6-siRNA. Asterisks indicate the centrosome. ( C ) Quantification of A, B, and D as intensity of the signal at the nucleus normalized to siControl. Statistical assay: two-way ANOVA with post-hoc Bonferroni comparison. ****p<0.0001, n = 60, 60, 19, 28, 58, 46, 56, 46 (from left to right), data are pooled from three independent experiments. Error bars represent the SD. ( D ) Immunostaining of PCM1 (green), cardiac troponin I (magenta), and DNA (DAPI) in siControl- and siAKAP6-treated P3 cardiomyocytes. Scale bars: 10 µm. Figure 1—source data 1. Underlying data for panels D, F and G.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A–B ) Immunostaining of ( A ) Cdk5Rap2 (green) or ( B ) Pcnt (green) and AKAP9 (red) together with cardiac troponin I (magenta, cardiomyocyte-specific) and DNA (DAPI) in rat P3 cardiomyocytes transfected with control-siRNA or AKAP6-siRNA. Asterisks indicate the centrosome. ( C ) Quantification of A, B, and D as intensity of the signal at the nucleus normalized to siControl. Statistical assay: two-way ANOVA with post-hoc Bonferroni comparison. ****p<0.0001, n = 60, 60, 19, 28, 58, 46, 56, 46 (from left to right), data are pooled from three independent experiments. Error bars represent the SD. ( D ) Immunostaining of PCM1 (green), cardiac troponin I (magenta), and DNA (DAPI) in siControl- and siAKAP6-treated P3 cardiomyocytes. Scale bars: 10 µm. Figure 1—source data 1. Underlying data for panels D, F and G.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Immunostaining, Transfection

( A ) Immunostaining of α-tubulin (green), cardiac troponin I (red, cardiomyocyte-specific), and DNA (DAPI) in siControl- or siAKAP6-treated P3 cardiomyocytes. Arrows denote the perinuclear microtubule cage in siControl cells, while arrowheads denote disorganized microtubules. ( B ) Quantification of A as α-tubulin intensity in concentric bands around the nucleus normalized to the total intensity of α-tubulin in the cell, 119 siControl cells and 131 siAKAP6 cells were quantified per condition from four independent experiments. Error bars represent the SD. ( C ) Immunostaining of α-tubulin (green), GM130 (red), AKAP9 (magenta), and DNA (DAPI) in siControl-, siAKAP6, and siAKAP6 + siAKAP9-treated P3 cardiomyocytes after 2 min of recovery from nocodazole-induced microtubule depolymerization, indicating a switch of MTOC activity from nuclear envelope to Golgi upon AKAP6 depletion (arrowheads). ( D ) Quantification of α-tubulin intensity in 0.5 µm wide concentric bands in AKAP6-depleted cells, as in C. 37 siControl cells and 48 siAKAP6 cells were quantified per condition from three independent experiments. Error bars represent the SD. ( E ) Staining of γ-tubulin (magenta) in siControl- or siAKAP6-treated cells. ( F–G ) Immunostaining of ( F ) ninein (magenta) and AKAP9 (green) in AKAP6-depleted cells or ( G ) ninein (magenta) and Pcnt (green) in AKAP9-depleted cells. Scale bars: 10 µm. Figure 4—source data 1. Underlying data for graphs in panels B and D.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A ) Immunostaining of α-tubulin (green), cardiac troponin I (red, cardiomyocyte-specific), and DNA (DAPI) in siControl- or siAKAP6-treated P3 cardiomyocytes. Arrows denote the perinuclear microtubule cage in siControl cells, while arrowheads denote disorganized microtubules. ( B ) Quantification of A as α-tubulin intensity in concentric bands around the nucleus normalized to the total intensity of α-tubulin in the cell, 119 siControl cells and 131 siAKAP6 cells were quantified per condition from four independent experiments. Error bars represent the SD. ( C ) Immunostaining of α-tubulin (green), GM130 (red), AKAP9 (magenta), and DNA (DAPI) in siControl-, siAKAP6, and siAKAP6 + siAKAP9-treated P3 cardiomyocytes after 2 min of recovery from nocodazole-induced microtubule depolymerization, indicating a switch of MTOC activity from nuclear envelope to Golgi upon AKAP6 depletion (arrowheads). ( D ) Quantification of α-tubulin intensity in 0.5 µm wide concentric bands in AKAP6-depleted cells, as in C. 37 siControl cells and 48 siAKAP6 cells were quantified per condition from three independent experiments. Error bars represent the SD. ( E ) Staining of γ-tubulin (magenta) in siControl- or siAKAP6-treated cells. ( F–G ) Immunostaining of ( F ) ninein (magenta) and AKAP9 (green) in AKAP6-depleted cells or ( G ) ninein (magenta) and Pcnt (green) in AKAP9-depleted cells. Scale bars: 10 µm. Figure 4—source data 1. Underlying data for graphs in panels B and D.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Immunostaining, Activity Assay, Staining

( A ) Immunostaining of α-tubulin (red) and DAPI in P3 cardiomyocytes transfected with GFP-AKAP6-SR1-3 or GFP-AKAP6-SR1 after 2 min of recovery from nocodazole-induced microtubule depolymerization. ( B ) Immunostaining of α-tubulin (green), Pcnt (red), AKAP9 (magenta), and DNA (DAPI) in the indicated siRNA-treated cardiomyocytes after 2 min of recovery from nocodazole-induced microtubule depolymerization; insets: 5 µm. Asterisk indicates the centrosome and arrowheads indicate nuclear envelop MTOC. Scale bars: 10 µm.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A ) Immunostaining of α-tubulin (red) and DAPI in P3 cardiomyocytes transfected with GFP-AKAP6-SR1-3 or GFP-AKAP6-SR1 after 2 min of recovery from nocodazole-induced microtubule depolymerization. ( B ) Immunostaining of α-tubulin (green), Pcnt (red), AKAP9 (magenta), and DNA (DAPI) in the indicated siRNA-treated cardiomyocytes after 2 min of recovery from nocodazole-induced microtubule depolymerization; insets: 5 µm. Asterisk indicates the centrosome and arrowheads indicate nuclear envelop MTOC. Scale bars: 10 µm.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Immunostaining, Transfection

( A ) Immunostaining of AKAP6 (green) and GM130 (red) in human osteoclasts after 3, 6 or 7 days of differentiation. Scale bars: 10 µm. ( B ) Immunostaining of nesprin-1 (green), AKAP6 (magenta), α-tubulin (red), and DNA (DAPI) in control or AKAP6-depleted osteoclasts. Scale bars: 10 µm. ( C ) Quantification of B as nuclear intensity normalized to shControl. Error bars represent the SD. n = 48, 48, 34, 34 (from left to right). Data are pooled from three independent donors. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. ****: p<0.0001, n.s.: no significance. ( D–E ) Immunostaining of AKAP9 (green), Pcnt (magenta), α-tubulin (red), and DNA (DAPI) in control and AKAP6-depleted osteoclasts. Scale bars: 10 µm. ( E ) Quantification of nuclear intensity normalized to shControl. Error bars represent the SD. n = 77, 39, 77, 39, 58, 76 (from left to right). Data were pooled from three independent donors. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. ****p<0.0001. ( F ) Immunostaining of α-tubulin (green), EB1 (magenta), GM130 (red), and DNA (DAPI) in control and AKAP6-depleted osteoclasts, after 15 s of recovery from cold-induced microtubule depolymerization. Scale bars: 20 µm. Figure 6—source data 1. Underlying data for graphs in panels C and E.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A ) Immunostaining of AKAP6 (green) and GM130 (red) in human osteoclasts after 3, 6 or 7 days of differentiation. Scale bars: 10 µm. ( B ) Immunostaining of nesprin-1 (green), AKAP6 (magenta), α-tubulin (red), and DNA (DAPI) in control or AKAP6-depleted osteoclasts. Scale bars: 10 µm. ( C ) Quantification of B as nuclear intensity normalized to shControl. Error bars represent the SD. n = 48, 48, 34, 34 (from left to right). Data are pooled from three independent donors. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. ****: p<0.0001, n.s.: no significance. ( D–E ) Immunostaining of AKAP9 (green), Pcnt (magenta), α-tubulin (red), and DNA (DAPI) in control and AKAP6-depleted osteoclasts. Scale bars: 10 µm. ( E ) Quantification of nuclear intensity normalized to shControl. Error bars represent the SD. n = 77, 39, 77, 39, 58, 76 (from left to right). Data were pooled from three independent donors. Statistical test: two-way ANOVA with post-hoc Bonferroni comparison. ****p<0.0001. ( F ) Immunostaining of α-tubulin (green), EB1 (magenta), GM130 (red), and DNA (DAPI) in control and AKAP6-depleted osteoclasts, after 15 s of recovery from cold-induced microtubule depolymerization. Scale bars: 20 µm. Figure 6—source data 1. Underlying data for graphs in panels C and E.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Immunostaining

( A–B ) NIH3T3 fibroblasts co-transfected with mCherry-nesprin-1α, ( A ) FLAG-PACT-Pcnt or ( B ) FLAG-PACT-AKAP9, and GFP-AKAP6-SR1-3 and immunostained with anti-FLAG (magenta) and DAPI. Green arrowhead: centrosomal staining of FLAG-PACT. ( C–D ) Immunostaining of FLAG (green) in NIH3T3 fibroblasts co-transfected with Cherry-Farnesyl or Cherry-AKAP6-SR1-Farnesyl together with ( C ) FLAG-Pcnt-PACT or ( D ) FLAG-AKAP9-PACT indicating that farnesylated SR1 is sufficient to recruit PACT to the plasma membrane. Green arrowhead: centrosomal staining of FLAG-PACT-Pcnt. Scale bars: 10 µm.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A–B ) NIH3T3 fibroblasts co-transfected with mCherry-nesprin-1α, ( A ) FLAG-PACT-Pcnt or ( B ) FLAG-PACT-AKAP9, and GFP-AKAP6-SR1-3 and immunostained with anti-FLAG (magenta) and DAPI. Green arrowhead: centrosomal staining of FLAG-PACT. ( C–D ) Immunostaining of FLAG (green) in NIH3T3 fibroblasts co-transfected with Cherry-Farnesyl or Cherry-AKAP6-SR1-Farnesyl together with ( C ) FLAG-Pcnt-PACT or ( D ) FLAG-AKAP9-PACT indicating that farnesylated SR1 is sufficient to recruit PACT to the plasma membrane. Green arrowhead: centrosomal staining of FLAG-PACT-Pcnt. Scale bars: 10 µm.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Transfection, Staining, Immunostaining

( A–B ) ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP and immunostained for endogenous ( A ) Pcnt (magenta) or ( B ) AKAP9 (magenta) indicating that AKAP6 can recruit endogenous centrosomal proteins to the nuclear envelope of nesprin-1α-expressing cells. Note the partial nuclear localization of Pcnt or AKAP9 near the centrosome (asterisks). ( C–D ) Percentage of Cherry-nesprin-1α-AKAP6-GFP expressing cells showing non, partial, crescent or full recruitment of Pcnt ( C ) or AKAP9 ( D ) to the nuclear envelope. Error bars represent the SD. Data are pooled from four independent experiments. Total number of Cherry-nesprin-1α/AKAP6-GFP expressing cells analyzed are indicated. ( E ) ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP and immunostained for endogenous GM130 (magenta). Transfected cell is labeled with a yellow arrowhead. ( F ) Percentage of Cherry-nesprin-1α/AKAP6-GFP expressing cells showing non, partial, crescent or full recruitment of GM130 to the nuclear envelope. Data are represented as individual biological replicates, together with mean ± SD from three independent experiments. Total number of Cherry-nesprin-1α/AKAP6-GFP expressing cells analyzed are indicated. Scale bars: 10 µm. Figure 8—source data 1. Underlying data for graphs in panels C, D and F.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A–B ) ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP and immunostained for endogenous ( A ) Pcnt (magenta) or ( B ) AKAP9 (magenta) indicating that AKAP6 can recruit endogenous centrosomal proteins to the nuclear envelope of nesprin-1α-expressing cells. Note the partial nuclear localization of Pcnt or AKAP9 near the centrosome (asterisks). ( C–D ) Percentage of Cherry-nesprin-1α-AKAP6-GFP expressing cells showing non, partial, crescent or full recruitment of Pcnt ( C ) or AKAP9 ( D ) to the nuclear envelope. Error bars represent the SD. Data are pooled from four independent experiments. Total number of Cherry-nesprin-1α/AKAP6-GFP expressing cells analyzed are indicated. ( E ) ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP and immunostained for endogenous GM130 (magenta). Transfected cell is labeled with a yellow arrowhead. ( F ) Percentage of Cherry-nesprin-1α/AKAP6-GFP expressing cells showing non, partial, crescent or full recruitment of GM130 to the nuclear envelope. Data are represented as individual biological replicates, together with mean ± SD from three independent experiments. Total number of Cherry-nesprin-1α/AKAP6-GFP expressing cells analyzed are indicated. Scale bars: 10 µm. Figure 8—source data 1. Underlying data for graphs in panels C, D and F.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Transfection, Expressing, Labeling

( A–C ) Examples of partial, crescent or full recruitment of Pcnt ( A ), AKAP9 ( B ) or GM130 ( C ) of ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP. Scale bars: 10 µm.

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet: ( A–C ) Examples of partial, crescent or full recruitment of Pcnt ( A ), AKAP9 ( B ) or GM130 ( C ) of ARPE19 cells co-transfected with mCherry-nesprin-1α and AKAP6-GFP. Scale bars: 10 µm.

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Transfection

Journal: eLife

Article Title: AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9

doi: 10.7554/eLife.61669

Figure Lengend Snippet:

Article Snippet: Antibody , anti-AKAP9 (mouse monoclonal) , BD Biosciences , Cat# 611518, RRID: AB_398978 , IF: (1:250).

Techniques: Transfection, Construct, Sequencing, Negative Control, shRNA, Clone Assay, Modification, Software, Laser-Scanning Microscopy

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